mRNA in blood after 28 days
Feb 20, 2023
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- SARS-CoV-2 spike mRNA vaccine sequences circulate in blood up to 28 days after COVID-19 vaccination https://onlinelibrary.wiley.com/doi/1…
- Journal of Pathology, Microbiology and Immunology – the APMIS journal
- Copenhagen University Hospital
CONFLICT OF INTEREST
- Authors have no conflicts of interest to declare.
- Denmark used Pfizer-BioNTech (BTN162b2) Moderna (mRNA-1273)
- Both code for production of the full-length SARS-CoV-2 spike protein mRNA is encapsulated in lipid nanoparticles
- Non replicating
- The modified nucleotide sequences allow perfect identification of the vaccine sequences
- Patients with chronic hepatitis C virus (HCV) infection Received mRNA vaccinations
- To monitor HCV infection, RNA was extracted from patient plasma
- In 10 of 108 HCV patient samples, full-length or traces of SARS-CoV-2 spike mRNA vaccine sequences were found in blood, up to 28 days after COVID-19 vaccination.
- Upon intramuscular injection, the vaccine mRNA is taken up by muscle and immune cells, and transported to the regional lymph nodes, and concentrated in the spleen
- The vaccines consist of nonreplicating mRNA, expected to naturally decompose, both within the cytosol after translation and at the injection site.
- Half-life of mRNA translation, estimated from hours to a day.
- Translation is described to span up to 10 days
- The Infectious Diseases Society of America (IDSA)
- Vaccine mRNA is degraded quickly, by normal intracellular processes, there is no evidence for long-term detection of mRNA vaccines
- Method used in Denmark Genotyping whole RNA genome sequencing NCH and SARS-CoV-2 mRNA, directly from plasma samples
- We describe the unexpected finding of SARS-CoV-2 vaccine mRNA sequences
- Five consecutive sequencing runs (May 2021 to the end of June 2021)
- Five negative controls and five HCV-positive controls
- Both mRNA vaccine sequences have been modified and are only ~70% identical to the spike reference genome on a nucleotide level, making them distinct from circulating infectious SARS-CoV-2 sequences.
- Of the 108 patient samples, 10 samples (9.3%) had partial or up to full sequences of the vaccine mRNA sequence
- Analysis of mRNA vaccine function has focused on the immune response, and on protection of vaccinated individuals
- The LNPs have been reported to be rapidly cleared by immune cells, and mRNA rapidly degraded
- We expect that vaccine mRNA detected in plasma is contained within LNPs
- To our knowledge, our study is the first to detect Pfizer-BioNTech and Moderna COVID-19 mRNA vaccine sequences in blood after vaccination, and therefore provides new knowledge regarding the timeframe in which the mRNA can be detected.
- A future prospective study to establish the half-life of mRNA vaccines in vaccine recipients could be performed (using mRNA vaccine-specific PCRs)
Originally posted at Dr Campbells YouTube Channel